Fraud identification in fishmeal using polymerase chain reaction (PCR)

fraud identification of cow’s milk in buffalo’s milk and it’s products using the polymerase chain reaction

conclusions to avoid unfair competition and to assure consumers of accurate labeling, using this method is recommended for regulatory agencies. results the limit of detection of cow’s milk in buffalo’s milk, yogurt and cheese is 1, 2 and 4%, respectively. undeclared presence of cow’s milk was detected in 70% of the milk samples, 64% of the yogurt and 52% of the cheese samples. in 10% of the yog...

Polymerase Chain Reaction (PCR)

The polymerase chain reaction (PCR) is a powerful and widely used technique that has greatly advanced our ability to analyze genes. Genomic deoxyribonucleic acid (DNA) present in cells contains many thousands of genes. This makes it difficult to isolate and analyze any individual gene. PCR allows specific DNA sequences, usually corresponding to genes or parts of genes, to be copied from genomic...

Identification of Meat Species by Polymerase Chain Reaction ( PCR ) Technique

The origin of horse, dog, cat, bovine, sheep, porcine, and goat meat was determined by the polymerase chain reaction (PCR) technique, using species-specific primers. Test mixtures of meat were prepared by adding 5%, 2.5%, 1%, 0.5%, and 0.1% levels of pork, horse, cat, or dog meat to beef, sheep, and goat meat. Samples taken from those combinations were analyzed by PCR for species determination....

identification of the propionibacterium acnes using polymerase chain reaction (pcr) in the acne vulgaris lesions

background: acne vulgaris is an inflammatory chronic disease of pilosebaceous unit. one of the most important factors playing a role in occurrence of acne is presence of propionibacterium acnes. with the aim of molecular identification of the p. acnes from the acne vulgaris lesions, current research was carried out. methods: in this study, contents within the lesions was collected from 70 patie...

Quantitative Real-time Polymerase Chain Reaction (qRT-PCR). Fatemeh Mehrpooyan*